Application notes

Chromatin immunoprecipitation (ChIP)

ChIP protocol for A. thaliana using GFP-Trap

The protocol can be applied for all kinds of A. thaliana starting material such as seedlings, leaves and inflorescences.

ChIP protocol for A. thaliana using GFP-Trap (PDF)

ChIP with RFP-tagged proteins

RFP-Trap for ChIP analysis in transiently expressing a mCherry fusion in mammalian cells.

ChIP with RFP-tagged proteins (PDF)

CRISPR

Spot-System for CRISPR’ed Spot-Tag

Spot-Trap and GFP-Trap for capture and Spot-Label for detection of CRISPR’ed Spot-Tag and EGFP.

Spot-System for CRISPR’ed Spot-Tag (PDF)

Elution of GFP

Elution of GFP-fusion protein from the GFP-Trap

The GFP-Trap has a very high affinity and specificity. The GFP-Trap enables very effective pulldowns, but requires special protocols for elution of bound GFP-fusion proteins if they are not analyzed on-bead.

Elution of GFP-fusion protein from the GFP-Trap (PDF)

Enzyme activity assay

One step purification of GFP-tagged enzymes for enzymatic activity tests

Using the GFP-Trap, GFP-fusion proteins of interest can be efficiently immunoprecipitated from cellular extracts for subsequent assays.

One step purification of GFP-tagged enzymes for enzymatic activity tests (PDF)

Mass spectrometry

On-bead digest protocol for mass spectrometry

On-bead digest protocol for mass spectrometry following immunoprecipitation with GFP-Trap, RFP-Trap, Myc-Trap, Spot-Trap, etc..

On-bead digest protocol for mass spectrometry (PDF)

iST GFP-Trap Kit for mass spectrometry (application note)

GFP-Trap for Co-IP of GFP-fusion proteins plus their interaction partners and iST technology for sample preparation for MS analysis.

iST GFP-Trap Kit for mass spectrometry (PDF)

Protein arrays (bead-based)

GST- & GFP- nanobodies for bead-based protein arrays e.g. Luminex®

Bead-based protein arrays using GFP-VHH and GST-VHH for capture of GFP- and GST-fusion proteins and their interacting partners.

GST- & GFP- nanobodies for bead-based protein arrays (PDF)

Surface plasmon resonance (SPR)

Capture Surface for Biacore assays

GFP-VHH as capture surface reagent for direct immobilization of GFP-fusion proteins in BiacoreTM protein analysis assays.

Capture Surface for Biacore assays (PDF)

Ubiquitination

Ubiquitination of GFP-tagged proteins

GFP-Trap for the analysis of candidate ubiquitinated proteins under harsh conditions.

Ubiquitination of GFP-tagged proteins (PDF)